- 1(current)
Q. No.Consider the following statements:
| I: | Biolistics or gene gun can be used to introduce genes in both plant and animal cells. |
| II: | Agrobacterium tumefaciens does not naturally infect Monocots. |
| III: | Liposomes are used in gene therapy for cystic fibrosis. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Subtopic: Â Host & Desired DNA: II |Â Host & Desired DNA |
 74%
Level 2: 60%+
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
Subtopic: Â Selectable Markers: I |Â Selectable Markers: II |Â Host & Desired DNA |Â Tools |
 69%
Level 2: 60%+
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Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
Subtopic: Â Host & Desired DNA: II |Â Process of Biotech |Â Host & Desired DNA |
 53%
Level 3: 35%-60%
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DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments are cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is referred to as :
| 1. | restriction map | 2. | RFLP profile |
| 3. | F' factor | 4. | library |
Subtopic: Â Host & Desired DNA: II |Â Host & Desired DNA |
Level 3: 35%-60%
Hints
To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Codes:
| Column I | Column II | ||
| A. | Lysozyme | P. | Fungal cell |
| B. | Cellulase | Q. | Plant cell |
| C. | Chitinase | R. | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
Subtopic: Â Host & Desired DNA |
 92%
Level 1: 80%+
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- 1(current)
Q. No.
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