- 1(current)
Q. No.If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
Subtopic: Â Selection of Recombinant Transformants |
 85%
Level 1: 80%+
Hints
Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Restriction digestion of foreign DNA and plasmid vector
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A>B>C>E>D
2. A>B>E>C>D
3. A>C>B>E>D
4. A>D>E>B>C
A. Isolation of foreign DNA
B. Restriction digestion of foreign DNA and plasmid vector
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A>B>C>E>D
2. A>B>E>C>D
3. A>C>B>E>D
4. A>D>E>B>C
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Separation and Isolation of DNA fragments |Â Selection of Recombinant Transformants |Â Obtaining Copy of Gene from Donor DNA |
 84%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
The colonies of recombinant bacteria appear white because of:
| 1. | Insertional inactivation of alpha-galactosidase in non-recombinant bacteria |
| 2. | Insertional inactivation of beta-galactosidase in recombinant bacteria |
| 3. | Inactivation of glycosidase enzyme in recombinant bacteria |
| 4. | Non-recombinant bacteria containing beta-galactosidase |
Subtopic: Â Selectable Markers: III |Â Selection of Recombinant Transformants |Â Selectable Markers |
 82%
Level 1: 80%+
AIPMT - 2013
Hints
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table:
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table:
| Medium with Tetracycline | Medium with Ampicillin | |
| 1. | Growth | No growth |
| 2. | No growth | Growth |
| 3. | No growth | No growth |
| 4. | Growth | Growth |
Subtopic: Â Selection of Recombinant Transformants |
 81%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
- 1(current)
Q. No.
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