The cutting of DNA at specific locations became possible with the discovery of?

1. Restriction enzymes

2. Probes

3. Selectable markets

4. ligases

Subtopic: ย Tools: Enzymes: II |ย Tools |
ย 93%
Level 1: 80%+
NEET - 2015
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The DNA molecule to which the gene of interest is integrated for cloning is called:

1. Transformer 2. Vector
3. Template 4. Carrier
Subtopic: ย Tools: Vectors: I |ย Tools |
ย 85%
Level 1: 80%+
NEET - 2015
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The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Subtopic: ย Tools: Vectors: II |ย Tools |
ย 91%
Level 1: 80%+
NEET - 2015
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic: ย Tools: Vectors: I |ย Tools |
ย 81%
Level 1: 80%+
AIPMT - 2014
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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulase

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Subtopic: ย Obtaining Copy of Gene from Donor DNA |
ย 87%
Level 1: 80%+
AIPMT - 2013
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Subtopic: ย Separation and Isolation of DNA fragments |
ย 92%
Level 1: 80%+
AIPMT - 2013
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The colonies of recombinant bacteria appear white because of:

1. Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
2. Insertional inactivation of beta-galactosidase in recombinant bacteria
3. Inactivation of glycosidase enzyme in recombinant bacteria
4. Non-recombinant bacteria containing beta-galactosidase
Subtopic: ย Selectable Markers: III |ย Selection of Recombinant Transformants |ย Selectable Markers |
ย 82%
Level 1: 80%+
AIPMT - 2013
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During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic: ย General Design of an rDNA experiment |
ย 87%
Level 1: 80%+
NEET - 2013
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For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:

1. Silver or platinum

2. Platinum or zinc

3. Silicon or platinum

4. Gold or tungsten

Subtopic: ย Transforming Plant & Animal Cell |
ย 97%
Level 1: 80%+
AIPMT - 2012
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The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
Subtopic: ย Tools: Vectors: II |ย Cloning Vector |ย Tools |
ย 86%
Level 1: 80%+
AIPMT - 2012
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