In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
1. ligase is added.
2. the temperature of the apparatus is increased to 95 degree Celsius.
3. a suitable host cell is incubated with the fragments.
4. primers are added for annealing to DNA fragments.
Subtopic:  Process of Biotech |
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic:  Process of Biotech |
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Level 2: 60%+
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