Match the following columns and select the correct option.
| Column-I | Column-II | ||
| a. | Bt cotton | (i) | Gene therapy |
| b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
| c. | RNAi | (iii) | Detection of HIV infection |
| d. | PCR | (iv) | Bacillus thuringiensis |
| a | b | c | d | |
| 1. | (iii) | (ii) | (i) | (iv) |
| 2. | (ii) | (iii) | (iv) | (i) |
| 3. | (i) | (ii) | (iii) | (iv) |
| 4. | (iv) | (i) | (ii) | (iii) |
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (BT) is resistant to:
| 1. | Fungal diseases | 2. | Plant nematodes |
| 3. | Insect predators | 4. | Insect pests |
What triggers the activation of protoxin to active Bt toxin of Bacillus thuringiensis in bollworms?
1. Acidic pH of the stomach
2. Body temperature
3. Moist surface of midgut
4. Alkaline pH of the gut
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
| (a) | (b) | (c) | (d) | |
| 1. | (iv) | (iii) | (i) | (ii) |
| 2. | (iii) | (ii) | (iv) | (i) |
| 3. | (iii) | (iv) | (i) | (ii) |
| 4. | (ii) | (iv) | (iii) | (i) |
In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to:
Which of the following Bt crops is being grown in India by the farmers?
| 1. | Cotton | 2. | Brinjal |
| 3. | Soybean | 4. | Maize |
| 1. | Synthesis of cDNA from RNA using reverse transcriptase |
| 2. | Silencing of specific mRNA due to complementary RNA |
| 3. | Interference of RNA in the synthesis of DNA |
| 4. | Synthesis of mRNA from DNA |
| 1. | Tobacco budworm | 2. | Armyworm |
| 3. | Beetles | 4. | Mosquitoes |
| 1. | corn borer | 2. | cotton bollworms |
| 3. | aphids | 4. | flea hopper |
| 1. | B. thuringiensis forms protein crystals during a particular phase of its growth. |
| 2. | The protein crystals contain a toxic insecticidal protein. |
| 3. |
Once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the acidic pH of the gut.
|
| 4. | The activated toxin binds to the surface of midgut epithelial cells and creates pores that cause cell swelling and lysis and eventually cause the death of the insect. |