Match the following columns and select the correct option.
| Column-I | Column-II | ||
| a. | Bt cotton | (i) | Gene therapy |
| b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
| c. | RNAi | (iii) | Detection of HIV infection |
| d. | PCR | (iv) | Bacillus thuringiensis |
| a | b | c | d | |
| 1. | (iii) | (ii) | (i) | (iv) |
| 2. | (ii) | (iii) | (iv) | (i) |
| 3. | (i) | (ii) | (iii) | (iv) |
| 4. | (iv) | (i) | (ii) | (iii) |
| List-I | List-II | ||
| (A) | Gene therapy | (I) | Separation of DNA fragments |
| (B) | RNA interference | (II) | Diagnostic test for AIDS |
| (C) | ELISA | (III) | Cellular defense |
| (D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
| Options: | (A) | (B) | (C) | (D) |
| 1. | IV | I | II | III |
| 2. | IV | II | III | I |
| 3. | IV | III | II | I |
| 4. | IV | III | I | II |
| 1. | Enzyme Linked Immuno-Sorbent Assay (ELISA) technique |
| 2. | Recombinant DNA Technology |
| 3. | Serum and Urine analysis |
| 4. | Polymerase Chain Reaction (PCR) technique |
Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
| 1. | mutated gene does not appear on a photographic film as the probe has no complementarity with it. |
| 2. | mutated gene does not appear on a photographic film as the probe has complementarity with it. |
| 3. | mutated gene partially appears on a photographic film. |
| 4. | mutated gene completely and clearly appears on a photographic film. |
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
1. Purification of isolated protein
2. Detection of gene mutation
3. Molecular diagnosis
4. Gene amplification