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Q. No.In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Â Cloning Vector |
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From NCERT
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Consider the given two statements:
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
| I. | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene. |
| II. | When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors. |
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Â Cloning Vector |
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For their work in the discovery and characterization of restriction enzymes, the 1978 Nobel Prize for Physiology or Medicine was awarded to:
| 1. | Salvador Luria, Jean Weigle and Giuseppe Bertani |
| 2. | Nirenberg, Matthaei and Korana |
| 3. | Paul Berg, Stanley Cohen and Herbert Boyer |
| 4. | Werner Arber, Daniel Nathans, and Hamilton O. Smith |
Subtopic: Â Restriction Enzymes: Historical Background |
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Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
| II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Restriction Enzymes - Main Enzymes |
From NCERT
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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From NCERT
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Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
From NCERT
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Â Restriction Enzymes - Main Enzymes |
From NCERT
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Consider the given two statements:
| Assertion (A): | One antibiotic resistance gene of vector pBR322 helps in selecting the transformants and the other one in selecting recombinants. |
| Reason (R): | The non-recombinants can grow on both antibiotic-rich media while the recombinants grow only on single medium due to insertional inactivation. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Cloning Vector |
From NCERT
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Consider the given two statements:
| Assertion: (A) | We cannot see pure DNA fragments in the visible light under microscope. |
| Reason: (R) | The DNA fragments separate according to their shape only and are too small to be seen in the gel electrophoresis. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Separation and Isolation of DNA fragments |
From NCERT
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Which of the following is required in a cloning vector?
| 1. | Origin of replication, a selectable marker, and a resistance gene |
| 2. | Origin of replication, a selectable marker, and a foreign DNA insert |
| 3. | A gene for replication, a selectable marker, and an antibiotic resistance gene |
| 4. | Origin of replication, a high copy number, and an antibiotic resistance gene |
Subtopic: Â Cloning Vector |
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