| 1. | Virus | 2. | Phage | 
| 3. | Bacterium | 4. | Yeast | 
| Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. | 
| Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. | 
| 1. | Micropropagation | 
| 2. | Differentiation | 
| 3. | Somatic hybridization | 
| 4. | Totipotency | 
| 1. | Somatic embryos | 2. | Protoplasts | 
| 3. | Pollens | 4. | Callus | 
| List I | List II | ||
| A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm | 
| B. | Cry IAb | II. | ADA deficiency | 
| C. | Cry IAc | III. | Emphysema | 
| D. | Enzyme replacement therapy | IV. | Corn borer | 
| Statement I: | Two DNA sequences were prepared corresponding to A and B, chains of human insulin and were introduced in the plasmids of Agrobacterium to produce insulin chain. | 
| Statement II: | Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. | 
| Assertion (A): | Some ethical standards are required to evaluate the morality of scientific human activities that might help or harm living organisms. | 
| Reason (R): | Genetic Engineering Approval Committee make decisions regarding the validity of Genetically Modified (GM) research and the safety of introducing GM organisms for public services. | 
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). | 
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). | 
| 3. | (A) is True but (R) is False. | 
| 4. | (A) is False but (R) is True. | 
| 1. | Bt toxin requires acidic pH for activation | 
| 2. | Bacillus has protective mechanism against it | 
| 3. | In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form | 
| 4. | The protoxin requires protein conjugate for its activation |