- 1(current)
Q. No.In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Â Cloning Vector |
 51%
From NCERT
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Consider the given two statements:
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
| I. | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene. |
| II. | When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors. |
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Â Cloning Vector |
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Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
From NCERT
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Consider the given two statements:
| Assertion (A): | One antibiotic resistance gene of vector pBR322 helps in selecting the transformants and the other one in selecting recombinants. |
| Reason (R): | The non-recombinants can grow on both antibiotic-rich media while the recombinants grow only on single medium due to insertional inactivation. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Cloning Vector |
From NCERT
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Which of the following is required in a cloning vector?
| 1. | Origin of replication, a selectable marker, and a resistance gene |
| 2. | Origin of replication, a selectable marker, and a foreign DNA insert |
| 3. | A gene for replication, a selectable marker, and an antibiotic resistance gene |
| 4. | Origin of replication, a high copy number, and an antibiotic resistance gene |
Subtopic: Â Cloning Vector |
From NCERT
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Consider the given two statements:
| Assertion (A): | Plasmid DNA is used to monitor bacterial transformation with foreign DNA. |
| Reason (R): | Plasmid is extrachromosomal DNA in some bacteria. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True but (R) is False |
| 4. | (A) is False but (R) is True |
Subtopic: Â Cloning Vector |
From NCERT
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Â Cloning Vector |
 55%
From NCERT
NEET - 2023
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NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Plasmid |
| II: | Phage DNA |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Cloning Vector |
 54%
From NCERT
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- 1(current)
Q. No.
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