| 1. | The use of living cells and bacteria in industrial and scientific processes. | 
| 2. | The integration of natural sciences and organisms, cells, parts thereof, and molecular analogues for products and services. | 
| 3. | The use of biology to solve problems and make useful products. | 
| 4. | The use of biology to develop new products, methods and organisms intended to improve human health and society. | 
Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. | 
| 2. | developing methods and software tools for understanding biological data. | 
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). | 
| 4. | maintenance of sterile ambience in chemical engineering processes. | 
What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes | 
| II: | Inability of inclusion of desirable traits from other species | 
| 1. | Only I | 2. | Only II | 
| 3. | Both I and II | 4. | Neither I nor II | 
A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere | 
| 2. | It becomes a part of chromosome | 
| 3. | It does not contain any intron sequences | 
| 4. | It is a single stranded polynucleotide | 
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites | 
| 3. | Ori | 4. | Selectable marker | 
The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez | 
| 3. | Hershey and Chase | 4. | Cohen and Boyer | 
In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell | 
| 2. | links together newly joined fragments of DN | 
| 3. | makes millions of copies of a specific segment of DNA | 
| 4. | separates fragments of DNA by their length and electrical charges | 
| 1. | that reads same on the two strands when orientation of reading is kept the same. | 
| 2. | that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. | 
| 3. | that are complementary on the two strands when orientation of reading is kept the same. | 
| 4. | that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. | 
Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. | 
| 2. | They are produced by bacterial cells as a mechanism of self-defence. | 
| 3. | They digest DNA by removing nucleotides from a free 3' end. | 
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. | 
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII | 
| 3. | SmaI | 4. | BamHI |