Given below are two statements:
Statement I: Bt toxins are insect group specific and coded by a gene cry IAc.
Statement II: Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut.

In the light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are False
2. Statement I is True but Statement II is False
3. Statement I is False but Statement II is True
4. Both Statement I and Statement II are True
Subtopic:  Bt Crops |
 60%
Level 2: 60%+
NEET - 2024
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Match List-I with List-II
List-I List-II
A.  \(\alpha-1\) antitrypsin  I.  Cotton bollworm
B.  Cry IAb II.  ADA deficiency
C.  Cry IAc III.  Emphysema
D.  Enzyme replacement therapy IV.  Corn borer
Choose the correct answer from the options given below:
1. A-III, B-I, C-II, D-IV
2. A-III, B-IV, C-I, D-II
3. A-II, B-IV, C-I, D-III
4. A-II, B-I, C-IV, D-III
Subtopic:  Intro to Agri Applications | Bt Crops | Gene Therapy |
 83%
Level 1: 80%+
NEET - 2024
Hints

Bt toxin protein that can kill many insects, doesn't kill the Bacillus because:
1. Bt toxin requires acidic pH for activation
2. Bacillus has protective mechanism against it 
3. In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form
4. The protoxin requires protein conjugate for its activation
Subtopic:  Bt Crops |
 90%
Level 1: 80%+
NEET - 2024
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Following are the steps involved in action of toxin in Bt Cotton:
A. The inactive toxin converted into active form due to alkaline pH of gut of insect
B. Bacillus thuringiensis produce crystals with toxic insecticidal proteins.
C. The alkaline pH solubilises the crystals.
D. The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect.
E. The toxin proteins exist as inactive protoxins in bacteria.
Choose the correct sequence of steps from the options given below:
1. E→C→B→A→D
2. B→C→A→E→D
3. A→E→B→D→C
4. B→E→C→A→D
Subtopic:  Bt Crops |
 73%
Level 2: 60%+
NEET - 2024
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Match List-I with List-II:
List-I List-II
A. Genetically engineered Human Insulin I. Gene therapy
B. GM Cotton II. E.Coli
C. ADA Deficiency III. Antigen-antibody interaction 
D. ELISA IV. Bacillus thuringiensis
Choose the correct answer from the options given below:
1. A-III, B-II, C- IV, D- I
2. A-II, B-I, C- IV, D- III
3. A-IV, B-III, C- I, D- II
4. A-II, B-IV, C- I, D- III
Subtopic:  Bt Crops | Other GM Crops | Med Applications & Humulin | Gene Therapy | Molecular Diagnosis |
 90%
Level 1: 80%+
NEET - 2024
Hints

The Bt toxin in genetically engineered Bt cotton kills the pest by: 
1. creating pores in the midgut 
2. damaging the respiratory system 
3. degenerating the nervous system 
4. altering the pH of body fluids
Subtopic:  Bt Crops |
 93%
Level 1: 80%+
NEET - 2024
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Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (BT) is resistant to:

1. Fungal diseases 2. Plant nematodes
3. Insect predators 4. Insect pests
Subtopic:  Bt Crops |
 72%
Level 2: 60%+
NEET - 2020
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Match the organism with its use in biotechnology.

(a) Bacillus thuringiensis    (i) Cloning vector
(b) Thermus aquaticus  (ii) Construction of first rDNA molecule
(c) Agrobacterium tumefaciens  (iii) DNA polymerase
(d) Salmonella typhimurium  (iv) Cry proteins

Select the correct option from the following:

(a) (b) (c) (d)
1. (iv) (iii) (i) (ii)
2. (iii) (ii) (iv) (i)
3. (iii) (iv) (i) (ii)
4. (ii) (iv) (iii) (i)
Subtopic:  Bt Crops | Interference RNA Tech |
 90%
Level 1: 80%+
NEET - 2020
Hints

Match the following columns and select the correct option.

Column-I Column-II
a. Bt cotton (i) Gene therapy
b. Adenosine deaminase deficiency (ii) Cellular defense
c. RNAi  (iii) Detection of HIV infection
d. PCR  (iv) Bacillus thuringiensis
 
a b c d
1. (iii) (ii) (i) (iv)
2. (ii) (iii) (iv) (i)
3. (i) (ii) (iii) (iv)
4. (iv) (i) (ii) (iii)
Subtopic:  Bt Crops | Interference RNA Tech | Gene Therapy | Molecular Diagnosis |
 94%
Level 1: 80%+
NEET - 2020
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What triggers the activation of protoxin to active Bt toxin of Bacillus thuringiensis in bollworms?

1. Acidic pH of the stomach

2. Body temperature 

3. Moist surface of midgut

4. Alkaline pH of the gut

Subtopic:  Bt Crops |
 92%
Level 1: 80%+
NEET - 2019
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