- 1(current)
Q. No.Mark the first isolated restriction endonuclease enzyme
1. EcoR I
2. Bam HI
3. Hind II
4. EcoR II
Subtopic: Â Restriction Enzymes: Historical Background |
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Level 1: 80%+
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What is the significance of restriction enzymes recognizing specific palindromic sequences in recombinant DNA technology?
| 1. | It allows precise cutting at identical locations, creating complementary sticky ends for ligation. |
| 2. | It ensures that the DNA is cut at random sites to generate variability. |
| 3. | It prevents the host DNA from being degraded during the replication process. |
| 4. | It ensures that the DNA is replicated in a particular orientation within the host. |
Subtopic: Â Tools: Enzymes: I |Â Tools: Enzymes: II |Â Restriction Enzymes: Historical Background |
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Â Restriction Enzymes: Historical Background |Â Tools |
 89%
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Which of the following statements about restriction enzymes is incorrect?
| 1. | Restriction enzymes are also called molecular scissors because they cleave DNA at specific sequences. |
| 2. | They are naturally found in eukaryotic cells to protect against viral DNA. |
| 3. | EcoRI is a restriction enzyme that recognizes the sequence GAATTC. |
| 4. | Restriction enzymes often create sticky ends, facilitating ligation. |
Subtopic: Â Restriction Enzymes: Historical Background |Â Tools |
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
| 1. | 6 bp | 2. | 4 bp |
| 3. | 10 bp | 4. | 8 bp |
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Restriction Enzymes: Historical Background |
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Level 1: 80%+
NEET - 2024
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Match Column-I with Column-II and select the correct option:
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
| Column-I | Column-II | ||
| a. | Restriction endonuclease | (i) | Hind II |
| b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
| c. | First restriction endonuclease | (iii) | Isolated in 1963 |
| d. | EcoRI | (iv) | Developed in 1972 |
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Â Restriction Enzymes: Historical Background |Â Introduction & History |
 81%
Level 1: 80%+
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- 1(current)
Q. No.
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