| 1. | Manipulation of physical structures |
| 2. | Use of live organisms or enzymes for useful products and processes |
| 3. | Extraction of minerals from organisms |
| 4. | Creation of artificial intelligence |
| 1. | Techniques of using live organisms only |
| 2. | Techniques of using enzymes only to produce products and processes useful to humans |
| 3. | The integration of natural science and organisms, cells, parts there of and molecular analogues for product and services |
| 4. | Techniques which include only synthesising a gene and using it |
Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
| 1. | It enhances genetic diversity by introducing multiple random genes into the target organism. |
| 2. | It allows the isolation and introduction of only the desired gene(s) without the inclusion of unwanted genes. |
| 3. | It completely eliminates the possibility of mutations in the introduced genetic material. |
| 4. | It speeds up the hybridization process without requiring molecular cloning techniques. |
A kind of biotechnology involving manipulation of DNA is:
1. DNA replication
2. genetic engineering
3. denaturation
4. renaturation
| 1. | is not useful in production of improved variants. |
| 2. | preserves the genetic information in populations. |
| 3. | is a very fast process to produce large number of progeny in a very short time. |
| 4. | very often leads to inclusion and multiplication of undesirable genes along with the desired genes. |
What is true A,B and C in the given diagrammatic representation of rDNA technology ?

| I: | At A same restriction enzyme is used to cut both foreign and vector DNA |
| II: | The enzyme used at B is DNA ligase |
| III: | Step C can be called as transformation |
| 1. | I and II only |
| 2. | I and III only |
| 3. | II and III only |
| 4. | I,II and III |
| Column-I | Column-II | ||
| a. | Restriction endonuclease | (i) | Hind II |
| b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
| c. | First restriction endonuclease | (iii) | Isolated in 1963 |
| d. | EcoRI | (iv) | Developed in 1972 |