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Q. No.Isolation of genetic material from fungal cells involves the use of
1. Lysozyme
2. Cellulase
3. Chitinase
4. Polymerase
1. Lysozyme
2. Cellulase
3. Chitinase
4. Polymerase
Subtopic: Introduction & History: I | Introduction & History: II | Process of Biotech | Introduction & History |
94%
Level 1: 80%+
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Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?
1. Lysozyme, lipases, trypsin
2. Chitinase, cellulase, lysozyme
3. Chitinase, cellulase, trypsin
4. Trypsin, lipases, cellulase
Subtopic: Process of Biotech |
95%
Level 1: 80%+
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As the DNA is enclosed within the membrane so the membrane has to be broken to release DNA along with other macromolecules. It can be achieved by treating plant cell by
| 1. | Lysozyme | 2. | Lipases |
| 3. | Chitinase | 4. | Cellulase |
Subtopic: Process of Biotech | Separation and Isolation of DNA fragments |
82%
Level 1: 80%+
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
81%
Level 1: 80%+
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