Biolistic (gene gun) is suitable for

1.	disarming pathogen vectors
2.	transformation of plant cell
3.	constructing recombinant DNA by joining with vectors ​​​​​​​
4.	DNA fingerprinting

A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(1) b, a, c, d

(2) a, b, c, d

(3) c, b, a, d

(4) b, a, d, c

Which of the following DNA sequences qualifies to be designated as a palindrome?

1. 5 - GACCAG - 3’ in one strand

2. 3’- GACCAG - 5’ in one strand

3. 5’- GACGAG- 3’ 3’- CTGGTC - 5’

4. 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

pBR_322, which is frequently used as a vector for cloning gene in E. coli is a/an

1. original bacterial plasmid

2. modified bacterial plasmid

3. viral genome

4. transposon

The thermostable enzyme Taq polymerase used in PCR technique was discovered by

(1)Alec Jeffrey

(2)Karry Mullis

(3)Temin

(4)Baltimore

To be useful in genetic engineering a vector must have

1. An origin for replication
2. Low copy number
3. Low rates of multiplication
4. Many cloning sites for the same enzyme

 E.coli is  a commonly used host for gene cloning because

A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

Electroporation facilitates insertion of foreign DNA into the cell by

Alec Jeffrey’s name is associated with

a. DNA sequencing

b. DNA fingerprinting

c. RNA sequencing

d. Gene cloning