Q. No.DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
2. DNase I
3. RNase
4. Hind II
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water
Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain-
1. Lipase
2. protease
3. Exonuclease
4. Endonuclease
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
1. Plant cells- cellulase
2. Algae - Methylase
3. Fungi - Chitinase
4. Bacteria - Lysozyme
Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with
1. corn
2. rice
3. wheat
4. soy beans
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