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Q. No.The source of thermostable DNA polymerase used in Polymerase Chain Reaction is:
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
Subtopic: Polymerase Chain Reaction: PCR |
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
81%
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic: Process of Biotech |
79%
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic: Cloning Vector |
84%
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In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
| 1. | Pvu I | 2. | BamH I |
| 3. | Pst I | 4. | Pvu II |
Subtopic: Selectable Markers: III |
80%
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In a plasmid cloning vector what is responsible for controlling the copy number of the linked DNA?
1. Ori
2. MCS
3. Selectable marker
4. Promoter
1. Ori
2. MCS
3. Selectable marker
4. Promoter
Subtopic: Tools: Vectors: I |
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Consider the given two statements:
| Assertion (A): | The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. |
| Reason (R): | The normal E. coli cells do not carry resistance against any of these antibiotics. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Selectable Markers: I |
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In agarose gel electrophoresis the separated DNA fragments can be visualized only after staining the DNA with a compound known as:
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
Subtopic: Separation and Isolation of DNA fragments |
97%
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Biotechnology has been defined as ‘the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services, by:
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
Subtopic: Introduction & History: I |
90%
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
73%
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